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Catalog No: OAPC00081
Size:100 ul
Price: $390.00
SKU
OAPC00081
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for OAPC00081
Product Info
Predicted Species ReactivityHuman, Mouse, Rat, Cow
Product FormatLiquid. Contains 10 mM sodium azide.
ClonalityMonoclonal
IsotypeIgG1
HostMouse
ApplicationWB, IF
Additional InformationPackaging :
Liquid. Contains 10 mM sodium azide.
Reconstitution and StorageStore at -20C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
ImmunogenRecombinant full length human UCHL1 purified from E. coli.
PurificationUnpurified, concentrated culture supernatant.
Predicted Homology Based on Immunogen SequenceCow; Human; Mouse; Rat
SpecificitySpecific for the ~24kDa UCHL1 protein.
Application InfoThe antibody has been directly tested for reactivity in bovine, human, mouse and rat. It is expected that it will work on other mammalian tissues.
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 24k UCHL1 protein.
Rat spinal cord stained with anti-UCHL1(red) and anti-neurofilament NF-H antibody (cat. #OAPC00025)(green). The large cells are alpha -motorneurons and UCHL1 fills the cytoplasm of their perikaryaand dendrites.
Reference1. Doran JF, Jackson P, Kynoch PA, Thompson RJ. Isolation of PGP 9.5, a new human neurone-specific protein detected by high-resolution two-dimensional electrophoresis. J Neurochem. 40:1542-7 (1983).
2. Wilkinson KD, Lee KM, Deshpande S, Duerksen-Hughes P, Boss JM, Pohl J. The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase. Science. 1989 246:670-3 (1989).
3. Kurihara LJ, Kikuchi T, Wada K, Tilghman SM. Loss of Uch-L1 and Uch-L3 leads to neurodegeneration, posterior paralysis and dysphagia. Hum Mol Genet. 10:1963-70 (2001).
4. Liu Y, Fallon L, Lashuel HA, Liu Z, Lansbury PT Jr. The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility. Cell 111:209-18 (2002).
Gene SymbolUCHL1
Alias SymbolsNDGOA, PARK5, PGP95, SPG79, PGP9.5, Uch-L1, HEL-117, PGP 9.5, HEL-S-53
NCBI Gene Id7345
Protein NameUbiquitin carboxyl-terminal hydrolase isozyme L1
Description of TargetUbiquitin C-terminal hydrolase 1 (UCHL1) is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5 (1). It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein (2). The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice (3). Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease (4). Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker.
Uniprot IDP09936
Protein Accession #NP_004172
Nucleotide Accession #NM_004181
Molecular Weight~24kDa
  1. What is the species homology for "Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) (OAPC00081)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow".

  2. How long will it take to receive "Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) (OAPC00081)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) (OAPC00081)" provided in?

    This item is provided in "Liquid. Contains 10 mM sodium azide.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) (OAPC00081)"?

    This target may also be called "NDGOA, PARK5, PGP95, SPG79, PGP9.5, Uch-L1, HEL-117, PGP 9.5, HEL-S-53" in publications.

  5. What is the shipping cost for "Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) (OAPC00081)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) (OAPC00081)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) (OAPC00081)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "~24kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) (OAPC00081)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "UCHL1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "UCHL1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "UCHL1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "UCHL1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "UCHL1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "UCHL1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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