RPL22 Antibody (OAEB00219)

Catalog#: OAEB00219

• Size: 0.1mg

Human Cortex

• Gene Symbol: RPL22
• Protein Accession#: NP_000974.1
• Swissprot Id: P35268
• Host: Goat
• Application: IHC
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Predicted Homology Based on Immunogen Sequence: Bovine: 100%; Chicken: 100%; Dog: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 92%; Guinea pig: 84%; Horse: 84%
• Applications Info: Peptide ELISA: antibody detection limit dilution 1:16000.
  Western Blot: Preliminary experiments gave bands at approx 17-18kDa and 25kDa in Human Liver lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.8kDa according to NP_000974.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
• Peptide Sequence: C-SKESYELRYFQINQ
• Key Reference: * Anderson SJ, Lauritsen JP, Hartman MG, Foushee AM, Lefebvre JM, Shinton SA, Gerhardt B, Hardy RR, Oravecz T, Wiest DL.; Ablation of Ribosomal Protein L22 Selectively Impairs alphabeta T Development by Activation of a p53-Dependent Checkpoint.; Immunity. 2007 Jun;26(6):759-72. Epub 2007 Jun 7.; Pubmed 17555992
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.