Anti-RIP2 (human) (Nick-1) Antibody (OAED00310)

Catalog No: OAED00310

Size:100UG

Figure: Detection of endogenous human RIP2 in different human cell lines by the MAb to RIP2 (Nick-1). Method: Total cell extracts (50 ug) from Jurkat (lane1), THP-1 (lane 2) and HT1080 (lane 3) cell lines were run on a SDS-gel, and revealed with MAb to RIP2 (Nick-1) (1:1’000 in 5% milk powder) for 1 hour at rooom temperature followed by incubation with HRP-coupled anti-rat secondary antibody (1:4’000).

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: Nick-1
• Isotype: IgG2a
• Host: Rat
• Application: Western blot
• Reconstitution and Storage: Long Term Storage : -20C
  Short Term Storage : +4C
  Handling :
  Stable for at least 1 year after receipt when stored as recommended.
• Immunogen: Recombinant human RIP2 (receptor-interacting protein 2) (aa 1-322).
• Concentration: 1mg/ml
• Specificity: Recognizes human RIP2.
• Formulation: Liquid. In PBS containing 0.02% sodium azide.
• Application Info: Western Blot
• Reference: Participation of Rip2 in lipopolysaccharide signaling is independent of its kinase activity: C. Lu, et al.; J. Biol. Chem. 280, 16278 (2005), Abstract; Full Text
  Participation of Rip2 in LPS signaling is independent of its kinase activity: C. Lu, et al.; J. Biol. Chem. 280, 16278 (2005), Abstract; Full Text
  PIDD mediates NF-kB activation in Response to DNA damage: Janssens, et al.; Cell 123, 1079 (2005), Abstract;
  

Target Info

• Gene Symbol: RIPK2
• Gene Full Name: receptor interacting serine/threonine kinase 2
• Alias Symbols: CARD3;CARD-carrying kinase;CARD-containing IL-1 beta ICE-kinase;CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase;CARD-containing interleukin-1 beta-converting enzyme-associated kinase;CARDIAK;CCK;GIG30;growth-inhibiting gene 30;receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase;receptor-interacting protein 2;receptor-interacting serine/threonine-protein kinase 2;RICK;RIP2;RIP-2;RIP-like-interacting CLARP kinase;tyrosine-protein kinase RIPK2.
• NCBI Gene Id: 8767
• Protein Name: Receptor-interacting serine/threonine-protein kinase 2
• Description of Target: Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
• Uniprot ID: O43353