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Datasheets/Manuals | Printable datasheet for OAPC00018 |
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Guinea Pig |
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Product Format | Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
Clonality | Monoclonal |
Clone | 763 |
Isotype | IgG1 |
Host | Mouse |
Application | WB |
Additional Information | Packaging : 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
Reconstitution and Storage | Store at -20C; stable for at least 1 year. |
Immunogen | Peptide corresponding to amino acid residues from the N-terminal region of human retinoic acid receptor, alpha -isotype. |
Purification | Prepared from mouse ascites by ammonium sulfate precipitation followed affinity purification on a Protein G column. |
Predicted Homology Based on Immunogen Sequence | Cow; Dog; Guinea Pig; Human; Mouse; Rat |
Specificity | Specific for the ~48k RAR- alpha isotype. |
Application Info | The antibody has been directly tested for reactivity in Western blots in rat and human tissues. It is anticipated that the antibody will also work with bovine, canine, mouse and guinea pig tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Western blot of rat hippocampal lysate showing specific immunolabeling of the ~48k RAR- alpha protein. |
Reference | Abu J, Batuwangala M, Herbert K, Symonds P (2005) Retinoic acid and retinoid receptors: potential chemopreventive and therapeutic role in cervical cancer. Lancet Oncol 6:712-720. Joshi S, Guleria R, Pan J, Dipette D, Singh US (2005) Retinoic acid receptors and tissue-trans-glutaminase mediate short-term effect of retinoic acid on migration and invasion of neuroblastoma SH-SY5Y cells. Oncogene advance online publication 12 September 2005; doi: 10.1038/sj.onc.1209027. Lane MA, Bailey SJ (2005) Role of retinoid signalling in the adult brain. Prog Neurobiol 75:275-293. |
Gene Symbol | RARA |
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Alias Symbols | RAR, NR1B1 |
NCBI Gene Id | 5914 |
Protein Name | Retinoic acid receptor alpha |
Description of Target | Retinoic acid (RA; active metabolite of vitamin A) plays a prominent role in regulating the transition of proliferating precursor cells (such as carcinoma cells and neuronal precursors) to postmitotic differentiated cells (Joshi et al., 2005). The retinoid X receptors (RXRs) family (RXR alpha, beta and gamma), preferentially bind 9-cis-RA and regulate gene transcription by forming heterodimers with a second family of RA receptors. RAs have been suggested to potentially play a therapeutic role in cervical cancer (Abu et al., 2005). RAs are known to play key roles in neuronal development and an increasing body of evidence indicates that retinoid signaling may regulate synaptic plasticity and associated learning and memory behaviors (Lane and Bailey, 2005). |
Uniprot ID | P10276 |
Protein Accession # | NP_000955 |
Nucleotide Accession # | NM_000964 |
Molecular Weight | ~48kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-Retinoic Acid Receptor, Alpha-Isotype (OAPC00018)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Guinea Pig".
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How long will it take to receive "Anti-Retinoic Acid Receptor, Alpha-Isotype (OAPC00018)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Anti-Retinoic Acid Receptor, Alpha-Isotype (OAPC00018)" provided in?
This item is provided in "Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-Retinoic Acid Receptor, Alpha-Isotype (OAPC00018)"?
This target may also be called "RAR, NR1B1" in publications.
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What is the shipping cost for "Anti-Retinoic Acid Receptor, Alpha-Isotype (OAPC00018)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-Retinoic Acid Receptor, Alpha-Isotype (OAPC00018)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-Retinoic Acid Receptor, Alpha-Isotype (OAPC00018)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~48kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-Retinoic Acid Receptor, Alpha-Isotype (OAPC00018)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "RARA"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "RARA"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "RARA"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "RARA"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "RARA"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "RARA"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.