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Datasheets/Manuals | Printable datasheet for OAPC00035 |
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Predicted Species Reactivity | Mouse, Rat, Cow, Dog |
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Product Format | Liquid. 100 ul antibody vial in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
Clonality | Polyclonal |
Host | Rabbit |
Application | WB, IHC |
Additional Information | Packaging : 100 ul antibody vial in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
Reconstitution and Storage | Store at -20C; stable for at least 1 year |
Immunogen | Peptide from the N-terminus of the NR2A subunit of rat NMDA receptor. |
Purification | Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled. |
Predicted Homology Based on Immunogen Sequence | Cow; Dog; Mouse; Rat |
Specificity | Specific for the ~180k NR2A subunit of the NMDA receptor. |
Application Info | The antibody has been directly tested for reactivity in Western blots rat tissue. It is anticipated that the antibody will also react with bovine, canine, and mouse tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~ 180k NR2A subunit. Immunostaining of 21 DIV nucleofected mouse striatal neuron (green) co-cultured with cortical neurons showing nice punctate labeling of the N-terminal NR2A subunit (red) in both the medium spiny neurons and the large pyramidal cell in the upper right. Photo courtesy of Dr. A.J. Milnerwood, Dr. Lynn Raymond Lab, University of British Columbia. |
Reference | Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025. Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577. Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33. Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724. Lu W-Y, Xiong Z-G, Lei S, Orser BA, Browning MD, MacDonald JF (1999) G-protein coupled receptors act via protein kinase C and Src to regulate NMDA receptors. Nature Neurosci 2:331-338. Snell LD, Nunley KR, Lickteig RL, Browning MD, Tabakoff B, Hoffman PL (1996) Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion. Mol Brain Res 40:71-78. Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358. Note: Dr. Michael Browning, a co-author of four of the cited papers, is President and founder of PhosphoSolutions. |
Gene Symbol | GRIN2A |
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Alias Symbols | NR2A, GluN2A, NMDAR2A |
NCBI Gene Id | 24409 |
Protein Name | Glutamate receptor ionotropic, NMDA 2A |
Description of Target | The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and alpha -amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. |
Uniprot ID | Q00959 |
Protein Accession # | NP_036705.3 |
Molecular Weight | ~180kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-NMDA Receptor, NR2A Subunit N-terminus (OAPC00035)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse, Rat, Cow, Dog".
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How long will it take to receive "Anti-NMDA Receptor, NR2A Subunit N-terminus (OAPC00035)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Anti-NMDA Receptor, NR2A Subunit N-terminus (OAPC00035)" provided in?
This item is provided in "Liquid. 100 ul antibody vial in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-NMDA Receptor, NR2A Subunit N-terminus (OAPC00035)"?
This target may also be called "NR2A, GluN2A, NMDAR2A" in publications.
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What is the shipping cost for "Anti-NMDA Receptor, NR2A Subunit N-terminus (OAPC00035)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-NMDA Receptor, NR2A Subunit N-terminus (OAPC00035)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-NMDA Receptor, NR2A Subunit N-terminus (OAPC00035)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~180kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-NMDA Receptor, NR2A Subunit N-terminus (OAPC00035)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GRIN2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GRIN2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GRIN2A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GRIN2A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GRIN2A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GRIN2A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.