Il10 Antibody (OASA08669)

Catalog#: OASA08669

• Size: 0.1mg

• Description of Target: RABBIT ANTI RAT INTERLEUKIN-10
• Gene Symbol: Il10
• Alias Symbols: IL10X; Il10
• Protein Accession#: NP_036986.2
• Swissprot Id: P29456
• Host: Rabbit
• Protein Size (# AA): 178
• Specificity: IL-10
• Application: ELISA, WB
• Immunogen: The immunogen for anti-Il10 antibody: recombinant rat IL-10
• Product Format: Purified IgG - lyophilised
• Isotype: Polyclonal IgG
• Applications Info: ELISA:    0.5ug/ml
  Western Blotting:    0.1ug/ml - 0.2ug/ml
  Functional Assays:    0.5ug/ml - 0.9ug/ml
  
• Additional Information: Note: The addition of 0.09% sodium azide is recommended for long term storage.
  N.B. For functional studies do not add sodium azide.
  Preservative Stabilisers: None present
  
  Antiserum Preparation: Antisera to rat IL-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
  Approx Protein Conc: IgG concentration 1.0 mg/ml
  
  Buffer Solutions: Phosphate buffered saline pH7.4
• Reconstitution and Storage: Reconstitution: Reconstitute with 0.1 ml distilled water
  Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at -20^oC.
  
  This product should be stored undiluted.
  
  Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Product Protocol: Il10 antibody used to evaluate functional and genetic analysis of two CD8 T cell subsets defined by the level of CD45RC expression in the rat (OASA08669)

Specificity: IL-10
Product Page: Il10 antibody (OASA08669)
Antibody Pair Available: OASA08669 Capture OASA08670 Detection
Experiment Type: Cytokine analysis

Protocol:
At various times throughout the culture (36 and 60 h for anti-TCR stimulation, 72, 96, and 120 h for MLR) supernatants were removed and stored at −20°C for cytokine determination; cells were harvested and RNA purified for analysis of lymphokine gene expression by RT-PCR. Emmanuel et. al. measured IFN-γ, IL-2, and IL-10 production in the supernatant by specific ELISA.

Summary:
In the present study, Emmanuel et. al. demonstrated for the first time that CD45RC expression distinguishes two CD8 T cell subsets in the rat: CDR45RChigh and CD45RClow. Upon in vitro stimulation in an APC-independent system, CD45RChigh CD8 T cells produce mainly IL-2 and IFN-γ, while the CD45RClow CD8 T cells produce only IL-4, IL-10, and IL-13.

References:
1: Xystrakis E, Cavailles P, Dejean AS, Cautain B, Colacios C, Lagrange D, van de
Gaar MJ, Bernard I, Gonzalez-Dunia D, Damoiseaux J, Fournié GJ, Saoudi A.
Functional and genetic analysis of two CD8 T cell subsets defined by the level of
CD45RC expression in the rat. J Immunol. 2004 Sep 1;173(5):3140-7. PubMed PMID:
15322174.