Ighg1 Antibody (OASA05589)

Catalog#: OASA05589

• Size: 1mg

• Description of Target: RAT ANTI MOUSE IgG1
• Gene Symbol: Ighg1
• Alias Symbols: IgG1; Igh-4; VH7183; Ighg1
• Swissprot Id: P01869
• Host: Rat
• Protein Size (# AA): 393
• Specificity: IgG1
• Application: ELISA
• Clone: LO-MG1-13
• Immunogen: The immunogen for anti-Ighg1 antibody: purified mouse IgG1 from BALB/c mice
• Product Format: Purified IgG - liquid
• Isotype: IgG1
• Applications Info: ELISA : This antibody may be used as a coating antibody in a sandwich ELISA in combination with LO-MG1-2 (OASA05590P) as the detection reagent.
  
• Additional Information: Fusion Partners: Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
  Preparation: Purified IgG prepared by affinity chromatography
  
  Preservative Stabilisers: 0.09% - Sodium Azide
  Approx Protein Conc: IgG concentration 1 mg/ml
  
  Buffer Solutions: Phosphate buffered saline
• Reconstitution and Storage: Store at +4^oC or at -20^oC if preferred.
  
  This product should be stored undiluted.
  
  Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
• Key Reference: 1. Denis, O. et al. (1993) Resting B cells can act as antigen presenting cells in vivo and induce antibody responses. Int. Immunol. 5: 71-78.
  2. Soares, M. et al. (1992) Isotype characterization of rat preformed natural antibodies against guinea pig cells. Transplantation Proceedings 24: 51-452.

Product Protocol: Ighg1 antibody used to evaluate increased b cell proliferation and reduced ig production in dream transgenic mice (OASA05589)

Specificity: IgG1
Product Page: Ighg1 antibody (OASA05589)
Antibody Pair Available: OASA05589 Capture OASA05593 Detection
Experiment Type: Ig ELISA

Protocol:
Rat anti-mouse IgG1 (LO-MG1-13), IgG2a (LO-MG2a-9), IgG2b (LO-MG2b-1), IgG3 (LO-MG3-13), IgM (LO-MM-3), or IgE (LO-ME-3) Abs for coating of plates and rat anti-mouse/HRP (LO-MK-1) as secondary detection step were from Serotec. For IgE, the secondary detection step was done with biotin-conjugated rat anti-mouse IgE mAb (BD Pharmingen) and detected by streptavidin–HRP conjugate (Amersham).

Summary:
1. B cells were stimulated with LPS plus IL-4 for 5 d, and Ig concentration in culture supernatants was assessed by ELISA. Stimulation with LPS alone induces class switch recombination (CSR) and production of IgG2b and IgG3 isotypes in wild-type B cells, whereas activation with LPS together with IL-4 or IFN-γ induces CSR and production of respectively IgG1/IgE and IgG2a. 2. Magali et al. observed that Ig secretion was markedly reduced in culture supernatants from LPS plus IL-4 stimulated transgenic B cells and LPS plus IFN-γ. After LPS stimulation, however, this defect was less pronounced and only significant for IgM.

References:
1: Savignac M, Mellström B, Bébin AG, Oliveros JC, Delpy L, Pinaud E, Naranjo JR. Increased B cell proliferation and reduced Ig production in DREAM transgenic mice. J Immunol. 2010 Dec 15;185(12):7527-36. Epub 2010 Nov 8. PubMed PMID: 21059893.