Anti-Fto (Fat mass and obesity-associated Protein) (OAPC00112)

Catalog No: OAPC00112

Size:100 ul

Western blot of rat testes lysate showing specific immunolabeling of the ~ 58k Fto protein.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Clonality: Monoclonal
• Clone: 5-2H10
• Isotype: IgG
• Host: Mouse
• Application: WB, IF
• Additional Information: Packaging :
  100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Reconstitution and Storage: Store at -20C in undiluted aliquots; stable for up to one year after date of receipt. Avoid freeze/thaw cycles.
• Immunogen: Synthetic peptide from the N-terminal region of rat Fto.
• Purification: Protein G purified culture supernatant.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Specificity: Specific for the ~58 kDa Fto protein in Western blots of rat testes lysate. The antibody has also been used for immunocytochemistry with neuronal progenitor cells.
• Application Info: The antibody has been directly tested for reactivity in human, mouse and rat. The immunogen sequence has 100% homology with bovine, canine, horse, non-human primate and sheep.
  Western blot of rat testes lysate showing specific immunolabeling of the ~ 58k Fto protein.
  
• Reference: Fredriksson R, Hägglund M, Olszewski PK, Stephansson O, Jacobsson JA, Olszewska AM, Levine AS, Lindblom J, Schiöth HB (2008). The obesity gene, FTO, is of ancient origin, upregulated during food deprivation and expressed in neurons of feeding-related nuclei of the brain. Endocrinology 149: 2062.
  Fischer J, Koch L, Emmerling C, Vierkotten J, Peters T, Brüning JC, Rüther U. (2009). Inactivation of the Fto gene protects from obesity. Nature 458: 894.

Target Info

• Gene Symbol: FTO
• Alias Symbols: GDFD, ALKBH9, BMIQ14
• NCBI Gene Id: 79068
• Protein Name: Alpha-ketoglutarate-dependent dioxygenase FTO
• Description of Target: The FTO gene is the most robust gene for common obesity characterized to date. FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which is involved in the stimulation of food intake (Fredricksson R et al., 2008). Deletion analysis of FTO gene in mice showed that Fto is functionally involved in the control of both energy intake and energy expenditure (Fischer J et al., 2009)
• Uniprot ID: Q9C0B1
• Protein Accession #: NP_001073901.1
• Molecular Weight: ~58kDa