Anti-FasL (mouse) (H11) Antibody (OAED00293)

Catalog No: OAED00293

Size:100UG

Schematic structure of mouse FasL (peptide, aa. 196-220)

Figure: Detection of FasL in 293T cells transfected with a FasL expression plasmid (right panel). Mock-transfected cells (left panel). Method: Cells extracts from (2 x 106) cells transfected with a FasL-expression plasmid were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-FasL H11 antibody at 2 ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG (ALX-211-052) and a chemiluminescence detection system. Arrow indicates FasL.

Product Info

• Predicted Species Reactivity: Mouse
• Clonality: Monoclonal
• Clone: H11
• Isotype: IgG2a
• Host: Rat
• Conjugation: Unconjugated
• Application: Flow cytometry|Immunocytochemistry|Western blot
• Reconstitution and Storage: Long Term Storage : +4C
  Handling : Do not freeze/thaw.
  Stable for at least 6 months after receipt when stored at +4C undiluted.
• Immunogen: Synthetic peptide corresponding to aa 196-220 of mouse FasL (CD95L; APO-1L; CD178).
• Concentration: 1mg/ml
• Specificity: Recognizes mouse FasL.
• Formulation: Liquid. In PBS containing 0.02% sodium azide.
• Purity: ≥95% (SDS-PAGE).
• Application Info: Flow Cytometry
  Immunocytochemistry
  Western Blot
  For immunohistochemistry, we recommend MAb to FasL (A11) (Prod. No. ALX-804-009). For the detection of human FasL by flow cytometry or Western blot, we recommmend MAb to FasL (human) (Mike-2) (Prod. No. ALX-804-125).
• Reference: Transgenic expression of CD95 ligand on thyroid follicular cells confers immune privilege upon thyroid allografts: L. Tourneur, et al.; J. Immunol. 167, 1338 (2001), Abstract;
  CD4+ T cells reactivated with superantigen are both more sensitive to FasL-mediated killing and express a higher level of FasL: J.K. Wang, et al.; Cell. Immunol. 179, 153 (1997), Abstract;
  Characterization of the non-functional Fas ligand of gld mice: M. Hahne, et al.; Int. Immunol. 7, 1381 (1995), Abstract;
  Apoptosis by death factor: S. Nagata; Cell 88, 355 (1997), Abstract;
  A role for CD95 ligand in preventing graft rejection: D. Bellgrau, et al.; Nature 377, 630 (1995), Abstract;
  Fas ligand-induced apoptosis as a mechanism of immune privilege: T.S. Griffith, et al.; Science 270, 1189 (1995), Abstract;
  Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways: B. Lowin, et al.; Nature 370, 650 (1994), Abstract;
  Mature T cells of autoimmune lpr/lpr mice have a defect in antigen-stimulated suicide: H. Russell, et al.; PNAS 90, 4409 (1993), Abstract; Full Text

Target Info

• Gene Symbol: Fasl
• Gene Full Name: Fas ligand (TNF superfamily, member 6)
• Alias Symbols: APT1;APT1LG1;CD178;CD95;CD95 ligand;CD95L;CD95-L;F;Fa;Fas antigen ligand;Fas-L;Faslg;generalized lymphoproliferative disease;gld;Tnfs;Tnfsf6;Tnlg1a;tumor necrosis factor ligand 1a;tumor necrosis factor ligand superfamily member 6.
• NCBI Gene Id: 14103
• Protein Name: Tumor necrosis factor ligand superfamily member 6
• Description of Target: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:7511063). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:19794494, PubMed:7532682). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (PubMed:19794494). TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (PubMed:10779162). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (By similarity).
• Uniprot ID: P41047