- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for Anti-Estrogen receptor (h-151) Antibody (OAED00222) (OAED00222) |
---|
Predicted Species Reactivity | Human|Rat |
---|---|
Product Format | Liquid. PBS (pH 7.2) containing 50% glycerol and 0.09% sodium azide |
Clonality | Monoclonal |
Clone | h-151 |
Isotype | IgG1 |
Host | Mouse |
Application | Immunocytochemistry|Immunoprecipitation|Western blot |
Reconstitution and Storage | Store long term at -20C. Avoid freeze/thaw cycles. |
Immunogen | Synthetic peptide corresponding to the sequence of human Estrogen Receptor, conjugated to KLH. |
Purification | Protein G affinity purified. |
Concentration | 1 mg/ml |
Specificity | Recognizes human and rat Estrogen Receptor. Detects a band of ~67kDa by Western blot. |
Application Info | IP: 15 ug/mL WB: 1:250 |
Reference | Serum-dependent effects of tamoxifen and cannabinoids upon C6 glioma cell viability: C.J. Fowler, et al. ; Biochem. Pharmacol. 60, 1807 (2000), Application(s): WB using rat samples, Abstract; Rapid actions of estrogens in GH3/B6 pituitary tumor cells via a plasma membrane version of estrogen receptor-alpha: B. Gametchu, et al. ; Steroids 64, 13 (1999), Application(s): ICC using human & rat samples, Abstract; An N-terminal inhibitory function, IF, suppresses transcription by the A-isoform but not the B-isoform of human progesterone receptors: K.B. Horwitz, et al. ; J. Biol. Chem. 273, 5455 (1998), Application(s): WB using human samples, Abstract; AIB1, a steroid receptor coactivator amplified in breast cancer and ovarian cancer: P.S. Meltzer, et al. ; Science 277, 965 (1997), Application(s): WB using human samples, Abstract; |
Gene Symbol | ESR1 |
---|---|
Gene Full Name | estrogen receptor 1 |
Alias Symbols | E2 receptor alpha;ER;Era;ER-alpha;ESR;ESRA;estradiol receptor;estrogen nuclear receptor alpha;estrogen receptor;estrogen receptor alpha E1-E2-1-2;estrogen receptor alpha E1-N2-E2-1-2;ESTRR;NR3A1;nuclear receptor subfamily 3 group A member 1;oestrogen receptor alpha. |
NCBI Gene Id | 2099 |
Protein Name | Estrogen receptor |
Description of Target | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1. |
Uniprot ID | P03372 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "Anti-Estrogen receptor (h-151) Antibody (OAED00222)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Rat".
-
How long will it take to receive "Anti-Estrogen receptor (h-151) Antibody (OAED00222)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 week".
-
What buffer format is "Anti-Estrogen receptor (h-151) Antibody (OAED00222)" provided in?
This item is provided in "Liquid. PBS (pH 7.2) containing 50% glycerol and 0.09% sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-Estrogen receptor (h-151) Antibody (OAED00222)"?
This target may also be called "E2 receptor alpha;ER;Era;ER-alpha;ESR;ESRA;estradiol receptor;estrogen nuclear receptor alpha;estrogen receptor;estrogen receptor alpha E1-E2-1-2;estrogen receptor alpha E1-N2-E2-1-2;ESTRR;NR3A1;nuclear receptor subfamily 3 group A member 1;oestrogen receptor alpha." in publications.
-
What is the shipping cost for "Anti-Estrogen receptor (h-151) Antibody (OAED00222)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "Anti-Estrogen receptor (h-151) Antibody (OAED00222)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "Anti-Estrogen receptor (h-151) Antibody (OAED00222)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-Estrogen receptor (h-151) Antibody (OAED00222)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "ESR1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "ESR1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "ESR1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "ESR1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "ESR1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "ESR1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.