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Datasheets/Manuals | Printable datasheet for OAPC00094 |
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Predicted Species Reactivity | Human, Mouse, Rat |
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Product Format | Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
Clonality | Polyclonal |
Host | Rabbit |
Application | WB, IHC |
Additional Information | Packaging : 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
Reconstitution and Storage | Store at -20C; stable for at least one year. |
Immunogen | peptide from the human collagen I alpha 1 propeptide sequence. |
Purification | Affinity purified |
Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
Specificity | Specific for the propeptide portion of the ~180 kDa collagen I alpha 1 polypeptide in human lung fibroblast extract. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized. |
Application Info | The antibody has been directly tested for reactivity in human, mouse and rat. Based on the homology of the antigen this antibody is expected to recognize the collagen I alpha 1 polypeptide in most, if not all, species of mammals, birds, amphibians, and fishes. Western blot of rat lung lysate showing specific immunolabeling of the ~180 k collagen 1 IHC of fibrotic mouse lung tissue showing specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized. |
Reference | Byers PH (1989) Inherited disorders of collagen gene structure and expression. Am J Med Genet. 34(1):72-80. Cabral WA, Makareeva E, Colige A, Letocha AD, Ty JM, Yeowell HN, Pals G, Leikin S, Marini JC. (2005) Mutations near amino end of alpha1(I) collagen cause combined osteogenesis imperfecta/Ehlers-Danlos syndrome by interference with N-propeptide processing. J Biol Chem. 2005 May 13;280(19):19259-69. |
Gene Symbol | COL1A1 |
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Alias Symbols | OI1, OI2, OI3, OI4, EDSC, CAFYD, EDSARTH1 |
NCBI Gene Id | 1277 |
Protein Name | Collagen alpha-1(I) chain |
Description of Target | Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the must abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005). |
Uniprot ID | P02452 |
Protein Accession # | NP_000079.2 |
Molecular Weight | ~180kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)" provided in?
This item is provided in "Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)"?
This target may also be called "OI1, OI2, OI3, OI4, EDSC, CAFYD, EDSARTH1" in publications.
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What is the shipping cost for "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~180kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "COL1A1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "COL1A1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "COL1A1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "COL1A1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "COL1A1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "COL1A1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.