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Datasheets/Manuals | Printable datasheet for Anti-Caspase-9 Polyclonal Antibody (OAAI00148) |
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Predicted Species Reactivity | Human |
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Product Format | Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot |
Additional Information | Background: Caspase-9 belongs to a member of the cysteine-aspartic acid protease (Caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small. Those dimerize to form the active enzyme. Activation of procaspase-9 by Apaf-1 in the Cytochrome C/dATP-dependent pathway requires proteolytic cleavage to generate the mature caspase molecule. Once activated, caspase-9 can initiate a caspase cascade involving the downstream executioner caspase-3, -6 and -7. |
:: | Positive Control: Human liver tissue |
:: | Cellular Location: Cytoplasmic |
Reconstitution and Storage | The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles. |
Immunogen | A synthetic peptide derived from N-terminus of human Caspase 9 protein. |
Purification | Affinity Purified |
Concentration | 100ug/200ul |
Specificity | CASP9 |
Formulation | This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
Application Info | WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested |
Reference | Duan, H et al. ICE-LAP6, a novel member of the ICE/CED-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J. Biol. Chem. 271 (28), 16720-16724(1996). |
Gene Symbol | CASP9 |
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Gene Full Name | caspase 9 |
Alias Symbols | APAF3;APAF-3;apoptotic protease activating factor 3;apoptotic protease MCH-6;caspase 9, apoptosis-related cysteine peptidase;caspase-9;ICE-LAP6;ICE-like apoptotic protease 6;MCH6;PPP1R56;protein phosphatase 1, regulatory subunit 56. |
NCBI Gene Id | 842 |
Description of Target | Caspase-9 belongs to a member of the cysteine-aspartic acid protease (Caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small. Those dimerize to form the active enzyme. Activation of procaspase-9 by Apaf-1 in the Cytochrome C/dATP-dependent pathway requires proteolytic cleavage to geneRate the mature caspase molecule. Once activated, caspase-9 can initiate a caspase cascade involving the downstream executioner caspase-3, -6 and -7. |
Uniprot ID | P55211 |
Molecular Weight | 46 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-Caspase-9 Polyclonal Antibody (OAAI00148)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "Anti-Caspase-9 Polyclonal Antibody (OAAI00148)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Anti-Caspase-9 Polyclonal Antibody (OAAI00148)" provided in?
This item is provided in "Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-Caspase-9 Polyclonal Antibody (OAAI00148)"?
This target may also be called "APAF3;APAF-3;apoptotic protease activating factor 3;apoptotic protease MCH-6;caspase 9, apoptosis-related cysteine peptidase;caspase-9;ICE-LAP6;ICE-like apoptotic protease 6;MCH6;PPP1R56;protein phosphatase 1, regulatory subunit 56." in publications.
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What is the shipping cost for "Anti-Caspase-9 Polyclonal Antibody (OAAI00148)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-Caspase-9 Polyclonal Antibody (OAAI00148)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-Caspase-9 Polyclonal Antibody (OAAI00148)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "46".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-Caspase-9 Polyclonal Antibody (OAAI00148)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CASP9"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CASP9"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CASP9"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CASP9"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CASP9"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CASP9"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.