Anti-ARF1 (ARFS 3F1) Antibody (OAED00058)

Catalog No: OAED00058

Size:50 ug

Western Blot Analysis of ARF1: Lane 1: Lane 2: Rat Brain; Lane 3: Mouse Brain; Lane 4: MDCK.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Bovine, Dog, Chicken, Guinea Pig, Monkey, Pig, Rabbit, Sheep
• Clonality: Monoclonal
• Clone: ARFS 3F1
• Isotype: IgG2a
• Host: Mouse
• Application: WB
• Reconstitution and Storage: Long Term Storage : -20C
  
• Immunogen: Synthetic peptide corresponding to the sequence near the C-terminus of human ARF1.
• Specificity: Recognizes human, mouse, rat, bovine, dog, chicken, guinea pig, monkey, pig, rabbit and sheep ARF1. Detects a band of ~22kDa by Western blot.
• Formulation: Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
• Purity: Protein G affinity purified.
  
• Application Info: Western Blot (ECL, 1:250)
  Optimal conditions must be determined individually for each application.

Target Info

• Gene Symbol: ARF1
• Alias Symbols: PVNH8
• NCBI Gene Id: 375
• Protein Name: ADP-ribosylation factor 1
• Description of Target: ADP-ribosylation factors (ARF) are a ubiquitous family of GTP-binding proteins involved in membrane dynamics and vesicular trafficking. The ARF family also includes the structurally related ARF-like proteins. ARF family members possess a highly conserved structure and function in eukaryotes. ARFs function as essential proteins in Saccharomyces cerevisiae (ARF-1 and ARF-2) and in Drosophila melanogaster (ARF-like). Many studies associate ARF proteins with exocytic and endocytic transport pathways, and the activation of phospholipase D activity. ARF-1, an abundantly expressed Ras-like N-myristoylated GTP-binding protein, co-purifies with tightly bound GDP. ARF possesses an extra beta-strand (beta2E) and N-terminal helix, two structural elements missing in Ras. Like Ras, ARF-1 switches between the GTP-bound "on” state where it transfers signals to its effectors in the signaling pathway, and the GDP-bound "off” state.
• Uniprot ID: P84077