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- $55: Antibody & Protein in US
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| Datasheets/Manuals | Printable datasheet for OAPC00015 |
|---|
| Predicted Species Reactivity | Zebrafish |
|---|---|
| Product Format | Liquid containing 0.01% sodium azide. |
| Clonality | Monoclonal |
| Clone | C4 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | WB, IF, IHC |
| Additional Information | Packaging : Liquid containing 0.01% sodium azide. |
| Reconstitution and Storage | Store at -20C in undiluted aliquots; stable for at least one year. Avoid freeze/thaw cycles. |
| Immunogen | Chicken gizzard actin. |
| Purification | Unpurified ascites fluid. |
| Predicted Homology Based on Immunogen Sequence | Zebrafish |
| Specificity | Specific for the ~42 kDa actin protein in lysates from skeletal, cardiac, gizzard and aorta tissues. Reacts with all actin isoforms. |
| Application Info | The antibody reacts with all known actins from yeast to man. Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 42k actin protein. |
| Reference | Lessard, JL. Two monoclonal antibodies to actin: one muscle selective and one generally reactive. Cell Motility and Cytoskeleton (1998) 10:349-362. |
| Gene Symbol | ACTA1 |
|---|---|
| NCBI Gene Id | 421534 |
| Protein Name | Actin, alpha skeletal muscle |
| Description of Target | Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. |
| Uniprot ID | P68139 |
| Protein Accession # | NP_001026234.1 |
| Molecular Weight | ~42kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-Actin (OAPC00015)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Zebrafish".
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How long will it take to receive "Anti-Actin (OAPC00015)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Anti-Actin (OAPC00015)" provided in?
This item is provided in "Liquid containing 0.01% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-Actin (OAPC00015)"?
This target may also be called "" in publications.
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What is the shipping cost for "Anti-Actin (OAPC00015)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-Actin (OAPC00015)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-Actin (OAPC00015)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~42kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-Actin (OAPC00015)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ACTA1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ACTA1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ACTA1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ACTA1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ACTA1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ACTA1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
