ANO6 ELISA Kit (Human) (OKCD00984)

Catalog No: OKCD00984 (Formerly GWB-KBBEE2)

Size:96WELLS

Human ANO6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anoctamin 6 (ANO6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anoctamin 6 (ANO6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anoctamin 6 (ANO6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anoctamin 6 (ANO6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anoctamin 6 (ANO6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anoctamin 6 (ANO6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ANO6 Microplate: 96 Wells (12 x 8 Well Strips)
  • ANO6 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated ANO6 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.127 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Anoctamin 6 (ANO6). No significant cross-reactivity or interference between Anoctamin 6 (ANO6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ANO6
• Gene Full Name: anoctamin 6
• Alias Symbols: anoctamin-6;BDPLT7;SCAN channel;SCTS;small-conductance calcium-activated nonselective cation channel;TMEM16F;transmembrane protein 16F.
• NCBI Gene Id: 196527
• Protein Name: Anoctamin-6
• Description of Target: Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets and osteoblasts. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (By similarity). Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes.
• Uniprot ID: Q4KMQ2
• Protein Accession #: NP_001020527
• Nucleotide Accession #: NM_001025356.2
• Protein Size (# AA): 910