ANGPTL7 ELISA Kit (Mouse) (OKCD02366)

Catalog No: OKCD02366

Size:96WELLS

Mouse ANGPTL7 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	95-103%	95-103%	80-104%	90-101%
  EDTA Plasma (n=5)	97-105%	86-103%	88-98%	95-102%
  Heparin Plasma (n=5)	79-94%	80-101%	87-94%	97-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 7 (ANGPTL7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin Like Protein 7 (ANGPTL7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin Like Protein 7 (ANGPTL7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Angiopoietin Like Protein 7 (ANGPTL7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	93-104	101
  EDTA Plasma (n=5)	86-102	95
  Heparin Plasma(n=5)	90-98	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiopoietin Like Protein 7 (ANGPTL7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiopoietin Like Protein 7 (ANGPTL7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ANGPTL7 Microplate: 96 Wells (12 x 8 Well strips)
  • ANGPTL7 Lyophilized Standard: 2
  • 100X Biotinylated ANGPTL7 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.117 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Angiopoietin Like Protein 7 (ANGPTL7). No significant cross-reactivity or interference between Angiopoietin Like Protein 7 (ANGPTL7) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Angptl7
• Gene Full Name: angiopoietin-like 7
• Alias Symbols: angiopoietin-like protein 7;angiopoietin-related protein 7.
• NCBI Gene Id: 654812
• Protein Name: Angiopoietin-related protein 7
• Description of Target: Angiopoietin-related protein 7
• Uniprot ID: Q8R1Q3
• Protein Accession #: NP_001034643.1
• Nucleotide Accession #: NM_001039554.3
• Protein Size (# AA): 337