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Catalog No: OKEH03450
Size:96T
Price: $525.00
SKU
OKEH03450
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH03450
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ANGPTL4 ELISA Kit (Mouse) (OKEH03450) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Angptl4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Angptl4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Angptl4 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
ELISA Kit ReproducibilityMean Intra-assay CV%: <4.7% (n = 20)
Mean Inter-assay CV%: <7.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Angptl4 Microplate96 Wells (12 x 8 Well strips)
Angptl4 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Angptl4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.059 ng/mL
SpecificityNatural and recombinant Mouse Angiopoietin-related protein 4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAngptl4
Gene Full Nameangiopoietin-like 4
Alias Symbols425O18-1;angiopoietin-like protein 4;angiopoietin-related protein 4;Arp4;Bk89;fasting-induced adipose factor;FI;Fiaf;fibrinogen/angiopoietin-related protein;hepatic fibrinogen/angiopoietin-related protein;HF;Hfarp;major histocompatibility complex region NG27;NG2;Ng27;Pgar;Pgarg;Pp1158;secreted protein Bk89.
NCBI Gene Id57875
Protein NameQ9Z1P8
Description of TargetAngiopoietin-related protein 4
Uniprot IDhttps://www.uniprot.org/uniprot/Q9Z1P8
Protein Accession #NP_065606.2
Nucleotide Accession #NM_020581.2
Protein Size (# AA)410
Molecular Weight46 kDa
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