Catalog No: OKEH00016
Size:96T
Price: $475.00
SKU
OKEH00016
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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ANGPT2 ELISA Kit (Mouse) : 96 Wells (OKEH00016)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ANGPT2 ELISA Kit (Mouse) (OKEH00016) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANGPT2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANGPT2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ANGPT2 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 7.3% (n = 20)
ELISA Kit Component
ComponentAmount
ANGPT2 Microplate96 Wells (12 x 8 Well strips)
ANGPT2 Lyophilized Standard2
100X Biotinylated ANGPT2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity7.85 pg/mL
SpecificityNatural and recombinant Mouse Angiopoietin-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAngpt2
Gene Full Nameangiopoietin 2
Alias SymbolsAgpt2;An;Ang;Ang2;Ang-2;angiopoietin-2.
NCBI Gene Id11601
Protein NameO35608
Description of TargetAngiopoietin-2
Uniprot IDhttps://www.uniprot.org/uniprot/O35608
Protein Accession #NP_031452
Nucleotide Accession #NM_007426
Protein Size (# AA)496
Molecular Weight57 kDa
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