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Datasheets/Manuals | Printable datasheet for anti-ANGPT2 antibody - OAAB00109 |
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Predicted Species Reactivity | Human, Mouse |
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Product Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | WB, IHC-P, FC |
Additional Information | Function: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Cellular Location: Secreted. |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This ANGPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 404-432 amino acids from the C-terminal region of human ANGPT2. |
Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
Predicted Homology Based on Immunogen Sequence | Human; Mouse |
Concentration | Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Application Info | FC: 1:25 WB: 1:2000 IHC-P: 1:50~100 WB: 1:1000 |
Reference | Morrissey, C., et al. Prostate 70(16):1799-1808(2010) Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) : Bento, C.F., et al. Exp. Physiol. 95(9):955-970(2010) Vrbacky, F., et al. Hematology 15(4):210-214(2010) Chen, J., et al. Biochem. Biophys. Res. Commun. 398(2):212-216(2010) |
Gene Symbol | ANGPT2 |
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Alias Symbols | ANG2, AGPT2 |
NCBI Gene Id | 285 |
Protein Name | Angiopoietin-2 |
Description of Target | The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. |
Uniprot ID | O15123 |
Protein Accession # | NP_001138.1 |
Molecular Weight | 57 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ANGPT2 Antibody - C-terminal region (OAAB00109)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse".
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How long will it take to receive "ANGPT2 Antibody - C-terminal region (OAAB00109)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "ANGPT2 Antibody - C-terminal region (OAAB00109)" provided in?
This item is provided in "Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ANGPT2 Antibody - C-terminal region (OAAB00109)"?
This target may also be called "ANG2, AGPT2" in publications.
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What is the shipping cost for "ANGPT2 Antibody - C-terminal region (OAAB00109)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ANGPT2 Antibody - C-terminal region (OAAB00109)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ANGPT2 Antibody - C-terminal region (OAAB00109)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "57 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ANGPT2 Antibody - C-terminal region (OAAB00109)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ANGPT2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ANGPT2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ANGPT2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ANGPT2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ANGPT2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ANGPT2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.