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Catalog No: OKBB00361
Size:96 Tests
Price: $583.00
SKU
OKBB00361
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Angiostatin K1-3 ELISA Kit (Human) (OKBB00361)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's human Angiostatin K1-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Angiostatin K1-3 has been precoated onto 96-well plates. Standards (NS0,V98-S355(K1-3)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiostatin K1-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Angiostatin K1-3 amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (ng/mL)1.093.686.961.133.757.04
St. Dev.0.0410.1550.320.0570.1990.415
%CV3.764.214.595.045.305.89
ELISA Kit Component
ComponentAmount
Lyophilized recombinant human P53 standard10 ng/tube x 2
Anti-human P53 Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti-human P53 antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Additional InformationRange: 156pg/ml-10,000pg/ml
::Principle: Aviva’s human Angiostatin K1-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Angiostatin K1-3 specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Angiostatin K1-3 amount of sample captured in plate.
::Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity (ED₅₀ =70 nM) relative to kringles 1-4 (ED₅₀ = 135 nM).
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample Typeserum, plasma and cell culture supernates
Sensitivity<10 pg/ml
Predicted Homology Based on Immunogen SequenceNo detectable cross-reactivity with any other cytokine.
Reference1. O’Relly MS, Holmgren L, Shin Y, Chen C, Rosenthal RA, Moses M et al. Angiostatin: a novel
angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma. Cell
1994; 79: 315–328
2. Cao Y, Ji RW, Davidson D, Schaller J, Marti D, Sohndel S et al. Kringle domains of human angiostatin.
JBC 1996; 271: 29461–29467
SpecificityNatural and recombinant human Angiostatin K1-3
ImmunogenExpression system for standard: NS0; Immunogen sequence: V98-S355 (K1-3)
DescriptionFor quantitative detection of human Angiostatin K1-3 in cell culture supernatants, serum and plasma(heparin, EDTA).
Gene SymbolPLG
Gene Full Nameplasminogen
Alias SymbolsAng K1-3
NCBI Gene Id5340
Protein NamePlasminogen
Description of TargetAng K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity (ED50 =70 nM) relative to kringles 1-4 (ED50 = 135 nM).
Uniprot IDP00747
Protein Accession #NP_000292.1
Nucleotide Accession #NM_000301.3
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