Catalog No: OKEH01050
Size:96T
Price: $600.00
SKU
OKEH01050
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology AMY1A ELISA Kit (Human) (OKEH01050) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human AMY1A antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated AMY1A and incubated. The AMY1A found in the sample or standards competes with the biotinylated AMY1A for limited binding sites on the immobilized anti-human AMY1A antibody. Excess unbound biotinylated AMY1A and sample or standard AMY1A is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated AMY1A captured in the well and inversely proportional to the amount of AMY1A which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 3.12-200ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 1% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 3.2% (n = 20) Mean Inter-assay CV%: < 7.1% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
Sensitivity | 1.56 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Alpha-amylase 1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Gene Symbol | AMY1A, AMY1B, AMY1C |
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Gene Full Name | amylase alpha 1A, amylase alpha 1B, amylase alpha 1C |
Alias Symbols | 1,4-alpha-D-glucan glucanohydrolase 1;alpha-amylase 1;alpha-amylase 1A;alpha-amylase 1B;alpha-amylase 1C;AMY1;amylase alpha 1A (salivary);amylase alpha 1B (salivary);amylase alpha 1C (salivary);amylase, salivary, alpha-1A;amylase, salivary, alpha-1B;amylase, salivary, alpha-1C;glycogenase;salivary alpha-amylase;salivary amylase alpha 1A;salivary amylase alpha 1B;salivary amylase alpha 1C. |
NCBI Gene Id | 276, 277, 278 |
Protein Name | P04745 |
Description of Target | Alpha-amylase 1, Alpha-amylase 1A, Alpha-amylase 1B, Alpha-amylase 1C |
Uniprot ID | https://www.uniprot.org/uniprot/P04745 |
Protein Accession # | NP_001008219.1 |
Nucleotide Accession # | NM_001008218.1 |
Protein Size (# AA) | 511 |
Molecular Weight | 58 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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