Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH01050
Size:96T
Price: $600.00
SKU
OKEH01050
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH01050
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AMY1A ELISA Kit (Human) (OKEH01050) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human AMY1A antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated AMY1A and incubated. The AMY1A found in the sample or standards competes with the biotinylated AMY1A for limited binding sites on the immobilized anti-human AMY1A antibody. Excess unbound biotinylated AMY1A and sample or standard AMY1A is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated AMY1A captured in the well and inversely proportional to the amount of AMY1A which was contained in the sample or standard.
ELISA Kit Range3.12-200ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 1%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.2% (n = 20)
Mean Inter-assay CV%: < 7.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-AMY1A Microplate96 Wells (12 x 8 Well strips)
AMY1A Lyophilized Standard2
Sample Diluent1 x 20 mL
100X AMY1A-Biotin Complex1 x 60 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity1.56 ng/mL
SpecificityNatural and recombinant Human Alpha-amylase 1
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolAMY1A, AMY1B, AMY1C
Gene Full Nameamylase alpha 1A, amylase alpha 1B, amylase alpha 1C
Alias Symbols1,4-alpha-D-glucan glucanohydrolase 1;alpha-amylase 1;alpha-amylase 1A;alpha-amylase 1B;alpha-amylase 1C;AMY1;amylase alpha 1A (salivary);amylase alpha 1B (salivary);amylase alpha 1C (salivary);amylase, salivary, alpha-1A;amylase, salivary, alpha-1B;amylase, salivary, alpha-1C;glycogenase;salivary alpha-amylase;salivary amylase alpha 1A;salivary amylase alpha 1B;salivary amylase alpha 1C.
NCBI Gene Id276, 277, 278
Protein NameP04745
Description of TargetAlpha-amylase 1, Alpha-amylase 1A, Alpha-amylase 1B, Alpha-amylase 1C
Uniprot IDhttps://www.uniprot.org/uniprot/P04745
Protein Accession #NP_001008219.1
Nucleotide Accession #NM_001008218.1
Protein Size (# AA)511
Molecular Weight58 kDa
Write Your Own Review
You're reviewing:AMY1A ELISA Kit (Human) (OKEH01050)
Your Rating
We found other products you might like!