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Catalog No: OKEH04473
Size:96T
Price: $525.00
SKU
OKEH04473
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Amy1 ELISA Kit (Mouse) (OKEH04473) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Amy1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Amy1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Amy1 captured in the well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.4% (n = 20)
Mean Inter-assay CV%: <= 8.0% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Amy1 Microplate96 Wells (12 x 8 Well Strips)
Amy1 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Amy1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.26 pg/mL
SpecificityNatural and recombinant Mouse Alpha-amylase 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAmy1
Gene Full Nameamylase 1, salivary
Alias Symbols1,4-alpha-D-glucan glucanohydrolase 1;alpha-amylase 1;alpha-amylase amy1a;Amy-;Amy-1;Amy1a;C030014B17Rik;salivary and hepatic alpha-amylase.
NCBI Gene Id11722
Protein NameP00687
Description of TargetAlpha-amylase 1
Uniprot IDhttps://www.uniprot.org/uniprot/P00687
Protein Accession #NP_001103975.1
Nucleotide Accession #NM_001110505.1
Protein Size (# AA)511
Molecular Weight58 KDa
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