PRKAB1 Antibody - N-terminal region (OAAB16785)

Catalog No: OAAB16785

Size:400ul

The anti-PRKAB1 Pab (Cat. # OAAB16785) is used in Western blot to detect PRKAB1 in Jurkat cell lysate (Lane 1) and mouse spleen tissue lysate (Lane 2).

PRKAB1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

Western blot analysis of anti-PRKAB1 Antibody (N-term) (Cat.# OAAB16785) in mouse heart lysates (35ug/lane). PRKAB1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: AMPK beta (PRKAB1) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This AMPK beta (PRKAB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-34 amino acids from the N-terminal region of human AMPK beta (PRKAB1).
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  PRKAB1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: PRKAB1
• Alias Symbols: AMPK, HAMPKb
• NCBI Gene Id: 5564
• Protein Name: 5'-AMP-activated protein kinase subunit beta-1
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9Y478
• Protein Accession #: NP_006244.2
• Molecular Weight: 30382 Da