Amphiregulin(AR) ELISA Kit (Human) (OKBB00394)

Catalog No: OKBB00394

Size:96 Wells

Human Amphiregulin(AR) ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human Amphiregulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Amphiregulin has been precoated onto 96-well plates. Standards(E.coli, S101-K198) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Amphiregulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Amphiregulin amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	115	351	633	104	339	620
  SD	6.67	15.1	29.75	8.94	24.75	32.24
  CV%	5.8	4.3	4.7	8.6	7.3	5.2

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human Amphiregulin Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human Amphiregulin Standard: 2 x 2 ng/tube
  • 100X Biotinylated Anti-Human Amphiregulin Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and human milk
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human Amphiregulin
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: S101-K198
• Description: For quantitative detection of human Amphiregulin in cell culture supernatants, serum, plasma(heparin, EDTA), saliva and human milk.

Target Info

• Gene Symbol: AREG
• Gene Full Name: amphiregulin
• Alias Symbols: A REG, AR, AREG, Colorectum cell derived growth factor, CRDGF, MGC13647, OTTHUMP00000219184, Schwannoma derived growth factor, SDGF
• NCBI Gene Id: 374
• Protein Name: Amphiregulin
• Description of Target: Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to 4 q 13.1. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the Epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th 2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.
• Uniprot ID: P15514
• Protein Accession #: NP_001648.1
• Nucleotide Accession #: NM_001657.3