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Catalog No: OKEH03066
Size:96T
Price: $525.00
SKU
OKEH03066
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AMH ELISA Kit (Rat) (OKEH03066) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Amh has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Amh is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Amh captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Component
ComponentAmount
Anti-Amh Microplate96 Wells (12 x 8 Well strips)
Amh Lyophilized Standard2
100X Biotinylated Amh Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.158 ng/mL
SpecificityNatural and recombinant Rat Muellerian-inhibiting factor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAmh
Gene Full Nameanti-Mullerian hormone
Alias SymbolsAnti - Mullerian hormone (Mulerian inhibiting substance);anti-Muellerian hormone;MIS;muellerian-inhibiting factor;muellerian-inhibiting substance.
NCBI Gene Id25378
Protein NameP49000
Description of TargetMuellerian-inhibiting factor
Uniprot IDhttps://www.uniprot.org/uniprot/P49000
Protein Accession #NP_037034.1
Nucleotide Accession #NM_012902.1
Protein Size (# AA)553
Molecular Weight59 kDa
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