Catalog No: OKCA00171
Size:96 Wells
Price: $645.00
SKU
OKCA00171
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AMBP ELISA Kit (Rat) : 96 Wells (OKCA00171)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:100105%99-109%
Serum (n=4)1:20096%92-99%
Serum (n=4)1:40095%90-98%
Serum (n=4)1:80089%84-94%
ELISA Kit PrincipleAviva Systems Biology AMBP ELISA Kit (Rat) (OKCA00171) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Rat AMBP and blocked. Standards or samples are added to the plate wells along with a horseradish peroxidase (HRP) conjugated antibody specific to AMBP and incubated. AMBP immobilized in the wells competes with the liquid phase AMBP contained in the samples or standards for binding with the HRP-Conjugated AMBP Antibody. Wells are washed removing unbound AMBP, unbound HRP-Conjugated AMBP Antibody or AMBP/HRP-Conjugated AMBP Antibody complex. HRP-Conjugated AMBP Antibody bound to AMBP immobilized in the well remains. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of HRP-Conjugated AMBP Antibody captured in the well and inversely proportional to the amount of AMBP which was contained in the sample or standard.
ELISA Kit Range1.563 ng/mL - 100 ng/mL
ELISA Kit Recovery
Sample 1
Sample TypeAverage RecoveryRange
Serum (n=5)91%87-95%
Sample 2
Sample TypeAverage RecoveryRange
EDTA Plasma (n=4)99%99-104%
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020202020
Mean (ng/ml)12.66612.60312.29412.83912.92712.888
SD0.0330.0530.0250.0340.0660.073
CV%3.9466.382.9094.1048.1018.954
ELISA Kit Component
ComponentAmount
AMBP Microplate96 Well (12 x 8 Well Strips)
AMBP Standard (Lyophilized)2 x 100 ng
100X HRP-Conjugate1 x 60 uL
HRP-Conjugate Diluent1 x 10 mL
Sample Diluent2 x 20 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates
Sensitivity< 0.649 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of
rat AMBP. No significant cross-reactivity or interference between rat AMBP and analogues was observed.
Assay InfoAssay Methodology: Quantitative Competitive Immunoassay
Gene SymbolAMBP
Gene Full Namealpha-1-microglobulin/bikunin precursor
Alias SymbolsUTI
NCBI Gene Id25377
Protein NameProtein AMBP
Description of TargetThis gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.
Uniprot IDQ64240
Protein Accession #NP_037033
Nucleotide Accession #NM_012901
Protein Size (# AA)349
Molecular Weight39 kDa
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