AMBP ELISA Kit (Mouse) : 96 Wells (OKCA00132)

Catalog No: OKCA00132

Size:96 Wells

Mouse AMBP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Serum (n=4)	1:100	104%	101-107%
  Serum (n=4)	1:200	96%	93-99%
  Serum (n=4)	1:400	94%	90-97%
  Serum (n=4)	1:800	91%	84-95%

• ELISA Kit Principle: Aviva Systems Biology AMBP ELISA Kit (Mouse) (OKCA00132) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Mouse AMBP and blocked. Standards or samples are added to the plate wells along with a horseradish peroxidase (HRP) conjugated antibody specific to AMBP and incubated. AMBP immobilized in the wells competes with the liquid phase AMBP contained in the samples or standards for binding with the HRP-Conjugated AMBP Antibody. Wells are washed removing unbound AMBP, unbound HRP-Conjugated AMBP Antibody or AMBP/HRP-Conjugated AMBP Antibody complex. HRP-Conjugated AMBP Antibody bound to AMBP immobilized in the well remains. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of HRP-Conjugated
• ELISA Kit Range: 1.563 ng/mL - 100 ng/mL
• ELISA Kit Recovery:

  Sample 1
  Sample Type	Average Recovery	Range
  Serum (n=5)	90%	87-93%

  Sample 2
  Sample Type	Average Recovery	Range
  EDTA Plasma (n=4)	97%	92-102%

• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	20	20	20	20	20	20
  Mean (ng/ml)	12.57	12.507	12.004	12.739	12.827	12.789
  SD	0.032	0.052	0.024	0.033	0.065	0.072
  CV%	3.927	6.361	2.86	4.085	8.082	8.935

• ELISA Kit Component:
  • Anti-AMBP Microplate: 96 Well (12 x 8 Well Strips)
  • AMBP Standard (Lyophilized): 2 x 100 ng
  • 100X HRP-Conjugate: 1 x 60 uL
  • HRP-Conjugate Diluent: 1 x 10 mL
  • Sample Diluent: 2 x 20 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: < 0.649 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of
  mouse AMBP. No significant cross-reactivity or interference between mouse AMBP and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Competitive Immunoassay

Target Info

• Gene Symbol: AMBP
• Gene Full Name: alpha 1 microglobulin/bikunin
• Alias Symbols: I, In, UT, UTI, uli, ASPI, HI-3, Itil, HI-30, Intin4, AI194774
• NCBI Gene Id: 11699
• Protein Name: Protein AMBP
• Description of Target: This gene encodes a fusion protein that undergoes proteolytic processing to generate two mature proteins: alpha-1-microglobulin (A1m) is a heme-binding plasma glycoprotein of the lipocalin superfamily of proteins that bind to hydrophobic molecules, whereas bikunin belongs to the superfamily of Kunitz-type protease inhibitors. The transgenic mice specifically lacking bikunin, but not A1m, exhibit female infertility and an increased sensitivity to lung metastasis.
• Uniprot ID: Q07456
• Protein Accession #: NP_031469
• Nucleotide Accession #: NM_007443
• Protein Size (# AA): 349
• Molecular Weight: 39 kDa