AMACR ELISA Kit (Human) : 96 Wells (OKEH00696)

Catalog No: OKEH00696

Size:96T

Human AMACR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology AMACR ELISA Kit (OKEH00696) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-AMACR antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated AMACR and incubated. The AMACR found in the sample or standards competes with the biotinylated AMACR for limited binding sites on the immobilized anti-AMACR antibody. Excess unbound biotinylated AMACR and sample or standard AMACR is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated AMACR captured in the well and inversely proportional to the amount of AMACR which was contained in the sample or standard.
  
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.2% (n = 20)
  Mean Inter-assay CV%: < 9.3% (n = 20)
• ELISA Kit Component:
  • Anti-AMACR Microplate: 96 Wells (12 x 8 Well strips)
  • AMACR Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X AMACR-Biotin Complex: 1 x 60 ul
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 1 ng/mL
• Specificity: Natural and recombinant Human Alpha-methylacyl-CoA racemase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AMACR
• Gene Full Name: alpha-methylacyl-CoA racemase
• Alias Symbols: 2-methylacyl-CoA racemase;alpha-methylacyl-CoA racemase;AMACRD;CBAS4;P504S;RACE;RM.
• NCBI Gene Id: 23600
• Protein Name: Q9UHK6
• Description of Target: Alpha-methylacyl-CoA racemase
• Uniprot ID: https://www.uniprot.org/uniprot/Q9UHK6
• Protein Accession #: NP_001161067.1
• Nucleotide Accession #: NM_001167595.1
• Protein Size (# AA): 382
• Molecular Weight: 42 kDa