ALPL ELISA Kit (Rat) (OKEH03286)

Catalog No: OKEH03286

Size:96T

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology’s ALPL ELISA Kit (Human) (OKEH03286) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALPL has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ALPL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALPL captured in the well.
• ELISA Kit Range: 3.12-200U/L
• ELISA Kit Component:
  • Anti-Alpl Microplate: 96 Wells (12 x 8 Well strips)
  • Alpl Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Alpl Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 1.62U/L
• Specificity: Natural and recombinant Rat Alkaline phosphatase, tissue-nonspecific isozyme
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Alpl
• Gene Full Name: alkaline phosphatase, biomineralization associated
• Alias Symbols: Akp2;alkaline phosphatase 2, liver;alkaline phosphatase liver/bone/kidney isozyme;alkaline phosphatase, liver/bone/kidney;alkaline phosphatase, tissue-nonspecific isozyme;AP-TNAP;PHOA;tissue-nonspecific alkaline phosphatase;Tissue-nonspecific ALP alkaline phosphatase;TNSALP.
• NCBI Gene Id: 25586
• Protein Name: P08289
• Description of Target: Alkaline phosphatase, tissue-nonspecific isozyme
• Uniprot ID: https://www.uniprot.org/uniprot/P08289
• Protein Accession #: NP_037191.1
• Nucleotide Accession #: NM_013059.1
• Protein Size (# AA): 524
• Molecular Weight: 58 kDa