Catalog No: OKEH04166
Size:96T
Price: $525.00
SKU
OKEH04166
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Alpi ELISA Kit (Rat) (OKEH04166) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Alpi has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Alpi is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alpi captured in the well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Component
ComponentAmount
Alpi Microplate96 Wells (12 x 8 Well Strips)
Alpi Standard2 x 2 ng
Sample Diluent1 x 20 mL
Alpi Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.88 ng/mL
SpecificityNatural and recombinant Rat Intestinal-type alkaline phosphatase 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolALPI
Gene Full Namealkaline phosphatase, intestinal
Alias SymbolsIntestinal alkaline phosphatase I.
NCBI Gene Id24197
Protein NameP15693
Description of TargetIntestinal-type alkaline phosphatase 1
Uniprot IDhttps://www.uniprot.org/uniprot/P15693
Protein Accession #NP_073156.3
Nucleotide Accession #NM_022665.3
Protein Size (# AA)540
Molecular Weight58 kDa
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