ALOX12 ELISA Kit (Human) (OKCD02356)

Catalog No: OKCD02356

Size:96WELLS

Human ALOX12 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-12-Lipoxygenase (ALOX12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Arachidonate-12-Lipoxygenase (ALOX12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arachidonate-12-Lipoxygenase (ALOX12) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arachidonate-12-Lipoxygenase (ALOX12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ALOX12 Microplate: 96 Wells (12 x 8 Well strips)
  • ALOX12 Lyophilized Standard: 2
  • 100X Biotinylated ALOX12 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.113 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Arachidonate-12-Lipoxygenase (ALOX12). No significant cross-reactivity or interference between Arachidonate-12-Lipoxygenase (ALOX12) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ALOX12
• Gene Full Name: arachidonate 12-lipoxygenase, 12S type
• Alias Symbols: 12-LOX;12S-lipoxygenase;12S-LOX;arachidonate (12S)-lipoxygenase;arachidonate (15S)-lipoxygenase;Arachidonate 12-lipoxygenase, 12S-type;arachidonate 15-lipoxygenase,15S-type;linoleate 13S-lipoxygenase;lipoxin synthase 12-LO;LOG12;platelet 12-LOX;platelet-type 12-lipoxygenase;platelet-type lipoxygenase 12;polyunsaturated fatty acid lipoxygenase ALOX12.
• NCBI Gene Id: 239
• Protein Name: Arachidonate 12-lipoxygenase, 12S-type
• Description of Target: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation.
• Uniprot ID: P18054
• Protein Accession #: NP_000688.2
• Nucleotide Accession #: NM_000697.2
• Protein Size (# AA): 663