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Catalog No: OKCD02305
Size:96WELLS
Price: $725.00
SKU
OKCD02305
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Datasheets/ManualsPrintable datasheet for ALDOB ELISA kit (Rat)
Product Info
Predicted Species ReactivityRat
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)87-101%88-95%78-101%91-99%
EDTA Plasma (n=5)92-101%92-105%96-103%93-101%
Heparin Plasma (n=5)87-95%84-98%78-99%80-89%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldolase B, Fructose Bisphosphate (ALDOB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldolase B, Fructose Bisphosphate (ALDOB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldolase B, Fructose Bisphosphate (ALDOB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aldolase B, Fructose Bisphosphate (ALDOB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78.12-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)79-9994
EDTA Plasma (n=5)79-9895
Heparin Plasma(n=5)92-10196
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aldolase B, Fructose Bisphosphate (ALDOB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aldolase B, Fructose Bisphosphate (ALDOB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ALDOB Microplate96 Wells (12 x 8 Well strips)
ALDOB Lyophilized Standard2
100X Biotinylated ALDOB Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity37 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Aldolase B, Fructose Bisphosphate (ALDOB). No significant cross-reactivity or interference between Aldolase B, Fructose Bisphosphate (ALDOB) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolALDOB
Gene Full Namealdolase, fructose-bisphosphate B
Alias SymbolsAldo2, Fructose-bisphosphate aldolase B, LIV10, Liver-type aldolase
Protein NameFructose-bisphosphate aldolase B
Uniprot IDP00884
Protein Accession #NP_036628.2
Nucleotide Accession #NM_012496.2
Protein Size (# AA)364
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