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Catalog No: OKEH02446
Size:96T
Price: $625.00
SKU
OKEH02446
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02446
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ALDH4A1 ELISA Kit (Human) (OKEH02446) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALDH4A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ALDH4A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALDH4A1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
ELISA Kit Component
ComponentAmount
ALDH4A1 Microplate96 Wells (12 x 8 Well strips)
ALDH4A1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ALDH4A1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.093 ng/mL
SpecificityNatural and recombinant Human Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolALDH4A1
Gene Full Namealdehyde dehydrogenase 4 family member A1
Alias Symbolsaldehyde dehydrogenase family 4 member A1;ALDH4;delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial;epididymis secretory sperm binding protein;L-glutamate gamma-semialdehyde dehydrogenase;mitochondrial delta-1-pyrroline 5-carboxylate dehydrogenase;P5C dehydrogenase;P5CD;P5CDh.
NCBI Gene Id8659
Protein NameP30038
Description of TargetDelta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/P30038
Protein Accession #NP_001154976.1
Nucleotide Accession #NM_001161504.1
Protein Size (# AA)563
Molecular Weight62 kDa
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