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Catalog No: OKEH04118
Size:96T
Price: $525.00
SKU
OKEH04118
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Alcam ELISA Kit (Rat) (OKEH04118) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Alcam has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Alcam is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alcam captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Component
ComponentAmount
Anti-Alcam Microplate96 Wells (12 x 8 Well Strips)
Alcam Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Alcam Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Rat CD166 antigen
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAlcam
Gene Full Nameactivated leukocyte cell adhesion molecule
Alias SymbolsActivated leukocyte cell adhesion molecule;CD166 antigen;HB2;KG-CAM;Protein MEMD;SB-10 antigen.
NCBI Gene Id79559
Protein NameO35112
Description of TargetCD166 antigen
Uniprot IDhttps://www.uniprot.org/uniprot/O35112
Protein Accession #NP_113941.1
Nucleotide Accession #NM_031753.1
Protein Size (# AA)583
Molecular Weight65 kDa
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