Catalog No: OKEH04309
Size:96T
Price: $550.00
SKU
OKEH04309
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Rat, Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Akt1 ELISA Kit (Rat) (OKEH04309) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Akt1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Akt1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Akt1 captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/ml | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-Assay Precision - < 4.6%, n = 20 Mean Inter-Assay Precision - < 7.8%, n = 20 | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids. | ||||||||||||||||||||||
Sensitivity | 0.078 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Rat RAC-alpha serine/threonine-protein kinase | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | Akt1 |
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Gene Full Name | AKT serine/threonine kinase 1 |
Alias Symbols | Akt;AKT1 kinase;murine thymoma viral (v-akt) oncogene homolog 1;PKB alpha;Protein kinase B;protein kinase B alpha;RAC protein kinase alpha RAC-PK alpha;RAC-alpha serine/threonine-protein kinase;RAC-PK-alpha;thymoma viral proto-oncogene 1;v-akt murine thymoma viral oncogene homolog 1. |
NCBI Gene Id | 24185 |
Protein Name | P47196 |
Description of Target | RAC-alpha serine/threonine-protein kinase |
Uniprot ID | https://www.uniprot.org/uniprot/P47196 |
Protein Accession # | NP_150233.1 |
Nucleotide Accession # | NM_033230.2 |
Protein Size (# AA) | 480 |
Molecular Weight | 56 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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