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Catalog No: OAAF00431 (Formerly GWB-ASB875)
Size:100 ug
Price: $344.00
SKU
OAAF00431
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for AKT1 Antibody (Bi-Phospho-Tyr437/434) (OAAF00431)
Product Info
Predicted Species ReactivityHuman|Mouse|Rat
Product FormatLiquid
ClonalityPolyclonal
HostRabbit
ApplicationEnzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Reconstitution and Storage-20°C
ImmunogenThe antiserum was produced against synthesized peptide derived from human AKT1/3 around the phosphorylation site of Tyr437/434.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
SpecificityAKT1/3 (Phospho-Tyr437/434) antibody detects endogenous levels of AKT1/3 only when phosphorylated at tyrosine 437.
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
DilutionThe optimal working dilution should be determined by the end user.
Application InfoWB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:20000
ReferenceJones P.F., Proc. Natl. Acad. Sci. U.S.A. 88:4171-4175(1991).
Matsubara A., Diabetologia 44:910-913(2001).
Coffer P.J., Eur. J. Biochem. 201:475-481(1991).
Gene SymbolAKT1|AKT2|AKT3
Gene Full NameAKT serine/threonine kinase 1|AKT serine/threonine kinase 2|AKT serine/threonine kinase 3
Alias SymbolsAKT;AKT1m;HIHGHH;MPPH;MPPH2;murine thymoma viral (v-akt) homolog-2;PKB;PKB alpha;PKB beta;PKB gamma;PKB-ALPHA;PKBB;PKBBETA;PKBG;PKB-GAMMA;PRKBA;PRKBB;PRKBG;protein kinase Akt-2;Protein kinase Akt-3;Protein kinase B;protein kinase B alpha;protein kinase B beta;Protein kinase B gamma;proto-oncogene c-Akt;putative v-akt murine thymoma viral oncoprotein 2;RAC;rac protein kinase alpha;rac protein kinase beta;RAC-ALPHA;RAC-alpha serine/threonine-protein kinase;RAC-BETA;RAC-beta serine/threonine-protein kinase;RAC-gamma;RAC-gamma serine/threonine protein kinase;RAC-gamma serine/threonine-protein kinase;RAC-PK-alpha;RAC-PK-beta;RAC-PK-gamma;serine-threonine protein kinase;STK-2;v-akt murine thymoma viral oncogene homolog 1;v-akt murine thymoma viral oncogene homolog 2;v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma);v-akt murine thymoma viral oncogene-like protein 1.
NCBI Gene Id10000|207|208
Protein NameRAC-alpha serine/threonine-protein kinase|RAC-gamma serine/threonine-protein kinase
Description of TargetAKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI(3)P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3. Phosphorylates STK3/MST2 at 'Thr-117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation. Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity. Phosphorylation of BAD stimulates its pro-apoptotic activity. Phosphorylates KAT6A at 'Thr-369' and this phosphorylation inhibits the interaction of KAT6A with PML and negatively regulates its acetylation activity towards p53/TP53.|AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
Uniprot IDP31749|Q9Y243
Protein Accession #NP_001193658.1
Molecular Weight55 kDa
  1. What is the species homology for "AKT1 Antibody (Bi-Phospho-Tyr437/434) (OAAF00431)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".

  2. How long will it take to receive "AKT1 Antibody (Bi-Phospho-Tyr437/434) (OAAF00431)"?

    This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".

  3. What buffer format is "AKT1 Antibody (Bi-Phospho-Tyr437/434) (OAAF00431)" provided in?

    This item is provided in "Liquid".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "AKT1 Antibody (Bi-Phospho-Tyr437/434) (OAAF00431)"?

    This target may also be called "AKT;AKT1m;HIHGHH;MPPH;MPPH2;murine thymoma viral (v-akt) homolog-2;PKB;PKB alpha;PKB beta;PKB gamma;PKB-ALPHA;PKBB;PKBBETA;PKBG;PKB-GAMMA;PRKBA;PRKBB;PRKBG;protein kinase Akt-2;Protein kinase Akt-3;Protein kinase B;protein kinase B alpha;protein kinase B beta;Protein kinase B gamma;proto-oncogene c-Akt;putative v-akt murine thymoma viral oncoprotein 2;RAC;rac protein kinase alpha;rac protein kinase beta;RAC-ALPHA;RAC-alpha serine/threonine-protein kinase;RAC-BETA;RAC-beta serine/threonine-protein kinase;RAC-gamma;RAC-gamma serine/threonine protein kinase;RAC-gamma serine/threonine-protein kinase;RAC-PK-alpha;RAC-PK-beta;RAC-PK-gamma;serine-threonine protein kinase;STK-2;v-akt murine thymoma viral oncogene homolog 1;v-akt murine thymoma viral oncogene homolog 2;v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma);v-akt murine thymoma viral oncogene-like protein 1." in publications.

  5. What is the shipping cost for "AKT1 Antibody (Bi-Phospho-Tyr437/434) (OAAF00431)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "AKT1 Antibody (Bi-Phospho-Tyr437/434) (OAAF00431)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "AKT1 Antibody (Bi-Phospho-Tyr437/434) (OAAF00431)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "55 kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "AKT1 Antibody (Bi-Phospho-Tyr437/434) (OAAF00431)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "AKT1|AKT2|AKT3"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "AKT1|AKT2|AKT3"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "AKT1|AKT2|AKT3"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "AKT1|AKT2|AKT3"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "AKT1|AKT2|AKT3"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "AKT1|AKT2|AKT3"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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