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Catalog No: OKEH04358
Size:96T
Price: $525.00
SKU
OKEH04358
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Akr1c6 ELISA Kit (Mouse) (OKEH04358) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Akr1c6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Akr1c6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Akr1c6 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 7.4% (n = 20)
Mean Inter-assay CV%: <= 9.6% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Akr1c6 Microplate96 Wells (12 x 8 Well Strips)
Akr1c6 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Akr1c6 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.089 ng/mL
SpecificityNatural and recombinant Mouse Estradiol 17 beta-dehydrogenase 5
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAkr1c6
Gene Full Namealdo-keto reductase family 1, member C6
Alias Symbols17-beta-HSD 5;17HSD type 5;3alph;3alpha-HSD;Akr1;Akr1c1;aldo-keto reductase family 1, member C1 (aldehyde reductase);estradiol 17 beta-dehydrogenase 5;estradiol 17-beta-dehydrogenase (A-specific);Hsd17b;Hsd17b5;hydroxysteroid 17-beta dehydrogenase 5.
NCBI Gene Id83702
Protein NameP70694
Description of TargetEstradiol 17 beta-dehydrogenase 5
Uniprot IDhttps://www.uniprot.org/uniprot/P70694
Protein Accession #NP_085114.1
Nucleotide Accession #NM_030611.3
Protein Size (# AA)323
Molecular Weight37 KDa
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