AKR1B10 ELISA Kit (Human) : 96 Wells (OKEH02121)

Catalog No: OKEH02121

Size:96T

Human AKR1B10 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology AKR1B10 ELISA Kit (Human) (OKEH02121) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for AKR1B10 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AKR1B10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AKR1B10 captured in the well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.3% (n = 20)
  Mean Inter-assay CV%: < 10.3% (n = 20)
• ELISA Kit Component:
  • Anti-AKR1B10 Microplate: 96 Wells (12 x 8 Well strips)
  • AKR1B10 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated AKR1B10 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.23 ng/mL
• Specificity: Natural and recombinant Human Aldo-keto reductase family 1 member B10
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AKR1B10
• Gene Full Name: aldo-keto reductase family 1 member B10
• Alias Symbols: AKR1B11;AKR1B12;aldo-keto reductase family 1 member B10;aldo-keto reductase family 1, member B10 (aldose reductase);aldo-keto reductase family 1, member B11 (aldose reductase-like);Aldose reductase-like;aldose reductase-like 1;aldose reductase-like peptide;aldose reductase-related protein;ALDRLn;ARL1;ARL-1;ARP;hARP;HIS;HSI;SI reductase;small intestine reductase.
• NCBI Gene Id: 57016
• Protein Name: O60218
• Description of Target: Aldo-keto reductase family 1 member B10
• Uniprot ID: https://www.uniprot.org/uniprot/O60218
• Protein Accession #: NP_064695.3
• Nucleotide Accession #: NM_020299.4
• Protein Size (# AA): 316
• Molecular Weight: 36 kDa