AIMP1 Antibody (OAGA00383)

Catalog No: OAGA00383

Size:100 ul

Sample(30 ?g of whole cell lysate)
A:H1299
B:Hep G2
12% SDS PAGE
OAGA00383 diluted at 1:1000

AIMP1 antibody detects SCYE1 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
AIMP1 antibody (OAGA00383) dilution: 1:500.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AIMP1(OAGA00383) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with(Red) at 1:2000.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq]
  Positive Controls: H1299, HepG2
  Cellular Localization: Nucleus, Cytoplasm, cytosol, Cytoplasmic vesicle, secretory vesicle, Secreted, Endoplasmic reticulum, Golgi apparatus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 263 of AIMP1 (Uniprot ID#Q12904)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.54 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: AIMP1
• Gene Full Name: Aminoacyl tRNA synthase complex-interacting multifunctional protein 1
• Alias Symbols: p43, HLD3, EMAP2, SCYE1, EMAPII
• NCBI Gene Id: 9255
• Protein Name: Aminoacyl tRNA synthase complex-interacting multifunctional protein 1
• Uniprot ID: Q12904
• Protein Accession #: NP_001135887.1
• Molecular Weight: 34 kDa