AIF1 ELISA Kit (Rat) (OKEH06407)

Catalog No: OKEH06407

Size:96T

Rat AIF1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Aif1 ELISA Kit (Rat) (OKEH06407) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Aif1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Aif1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Aif1 captured in the well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 106%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <= 4.3% (n = 20)
  Mean Inter-Assay Precision - <= 8.2% (n = 20)
• ELISA Kit Component:
  • H3F3C Microplate: 96 Wells (12 x 8 Well strips)
  • H3F3C Lyophilized Standard: 2
  • 100X Biotinylated H3F3C Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Rat Allograft inflammatory factor 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Aif1
• Gene Full Name: allograft inflammatory factor 1
• Alias Symbols: AIF-1;allograft inflammatory factor 1;balloon angioplasty responsive transcript;balloon angioplasty-responsive transcript 1;Bart1;BART-1;iba1;ionized calcium binding adapter molecule 1;Ionized calcium-binding adapter molecule 1;microglia response factor;mrf-1;protein BART-1.
• NCBI Gene Id: 29427
• Protein Name: P55009
• Description of Target: Allograft inflammatory factor 1
• Uniprot ID: https://www.uniprot.org/uniprot/P55009
• Protein Accession #: NP_001153119
• Nucleotide Accession #: NM_001159647