AGE ELISA Kit (Mouse) (OKEH04175)

Catalog No: OKEH04175

Size:96T

Mouse AGE ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology AGE ELISA Kit (Mouse) (OKEH04175) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AGE has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AGE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AGE captured in the well.
• ELISA Kit Range: 0.78-50ng/ml
• ELISA Kit Recovery: <= 5.2%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 7.4% (n = 20)
  Mean Inter-assay CV%: <= 9.8% (n = 20)
• ELISA Kit Component:
  • AGE Microplate: 96 Wells (12 x 8 Well Strips)
  • AGE Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • AGE Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.412 ng/mL
• Specificity: Natural and recombinant Mouse Advanced glycation end-product
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AGE
• Gene Full Name: Advanced glycation end-product
• Description of Target: An advanced glycation end-product (AGE) is the result of a chain of chemical reactions after an initial glycation reaction. The intermediate products are known, variously, as Amadori, Schiff base and Maillard products, named after the researchers who first described them. (The literature is inconsistent in applying these terms. For example, Maillard reaction products are sometimes considered intermediates and sometimes end products.) Side products generated in intermediate steps may be oxidizing agents (such as hydrogen peroxide), or not (such as beta amyloid proteins). "Glycosylation" is sometimes used for "glycation" in the literature, usually as 'non-enzymatic glycosylation'.
• Protein Size (# AA): 430
• Molecular Weight: 50 kDa