Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH04820
Size:96T
Price: $525.00
SKU
OKEH04820
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology ADRA1A ELISA Kit (Human) (OKEH04820) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADRA1A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADRA1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADRA1A captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
ADRA1A Microplate96 Wells (12 x 8 Well Strips)
ADRA1A Standard2
Sample Diluent1 x 20 mL
ADRA1A Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Alpha-1A adrenergic receptor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolADRA1A
Gene Full Nameadrenoceptor alpha 1A
Alias SymbolsADRA1C;ADRA1L1;alpha-1A adrenergic receptor;alpha-1A adrenoceptor;alpha-1A adrenoreceptor;ALPHA1AAR;alpha-1C adrenergic receptor;Alpha-adrenergic receptor 1c;G protein coupled receptor.
NCBI Gene Id148
Protein NameP35348
Description of TargetAlpha-1A adrenergic receptor
Uniprot IDhttps://www.uniprot.org/uniprot/P35348
Protein Accession #NP_000671.2
Nucleotide Accession #NM_000680.3
Protein Size (# AA)466
Molecular Weight51 kDa
Write Your Own Review
You're reviewing:ADRA1A ELISA Kit (Human) (OKEH04820)
Your Rating
We found other products you might like!