ADRA1A ELISA Kit (Human) (OKCD00827)

Catalog No: OKCD00827 (Formerly GWB-KBBNP2)

Size:96WELLS

Human ADRA1A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adrenergic Receptor Alpha 1A (ADRa1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adrenergic Receptor Alpha 1A (ADRa1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adrenergic Receptor Alpha 1A (ADRa1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adrenergic Receptor Alpha 1A (ADRa1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenergic Receptor Alpha 1A (ADRa1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenergic Receptor Alpha 1A (ADRa1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ADRA1A Microplate: 96 Wells (12 x 8 Well Strips)
  • ADRA1A Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated ADRA1A Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.119 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adrenergic Receptor Alpha 1A (ADRa1A). No significant cross-reactivity or interference between Adrenergic Receptor Alpha 1A (ADRa1A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ADRA1A
• Gene Full Name: adrenoceptor alpha 1A
• Alias Symbols: ADRA1C;ADRA1L1;alpha-1A adrenergic receptor;alpha-1A adrenoceptor;alpha-1A adrenoreceptor;ALPHA1AAR;alpha-1C adrenergic receptor;Alpha-adrenergic receptor 1c;G protein coupled receptor.
• NCBI Gene Id: 148
• Protein Name: Alpha-1A adrenergic receptor
• Description of Target: This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.
• Uniprot ID: P35348
• Protein Accession #: NP_000671
• Nucleotide Accession #: NM_000680.3
• Protein Size (# AA): 466