ADIPOR1 ELISA Kit (Mouse) (OKCD02347)

Catalog No: OKCD02347

Size:96 Wells

Mouse ADIPOR1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-91%	82-99%	79-101%	78-98%
  EDTA Plasma (n=5)	83-92%	98-105%	93-101%	96-103%
  Heparin Plasma (n=5)	91-98%	90-98%	85-103%	83-90%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin Receptor 1 (ADIPOR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adiponectin Receptor 1 (ADIPOR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adiponectin Receptor 1 (ADIPOR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adiponectin Receptor 1 (ADIPOR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-99	92
  EDTA Plasma (n=5)	93-102	98
  Heparin Plasma(n=5)	99-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adiponectin Receptor 1 (ADIPOR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adiponectin Receptor 1 (ADIPOR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ADIPOR1 Microplate: 96 Wells (12 x 8 Well strips)
  • ADIPOR1 Lyophilized Standard: 2
  • 100X Biotinylated ADIPOR1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 1.17 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adiponectin Receptor 1 (ADIPOR1). No significant cross-reactivity or interference between Adiponectin Receptor 1 (ADIPOR1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Adipor1
• Gene Full Name: adiponectin receptor 1
• Alias Symbols: 2810031L11Rik;ACDCR1;adiponectin receptor protein 1;CGI-45;Paqr1;progestin and adipoQ receptor family member 1;progestin and adipoQ receptor family member I.
• NCBI Gene Id: 72674
• Protein Name: Adiponectin receptor protein 1
• Description of Target: Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism (PubMed:17327425, PubMed:17268472, PubMed:24742672). Required for normal glucose and fat homeostasis and for maintaining a normal body weight (PubMed:17327425, PubMed:24742672). ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis (PubMed:12802337, PubMed:17327425, PubMed:17268472, PubMed:24742672). Has high affinity for globular adiponectin and low affinity for full-length adiponectin (PubMed:12802337).
• Uniprot ID: Q91VH1
• Protein Accession #: NP_001292998.1
• Nucleotide Accession #: NM_001306069.1
• Protein Size (# AA): 375