Adiponectin ELISA Kit (Human) (OKBB00291)

Catalog No: OKBB00291

Size:96 Tests

Human ADIPOQ ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Adiponectin has been precoated onto 96-well plates. Standards(NS0, E19-N244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Adiponectin amount of sample captured in plate.
• ELISA Kit Range: 1.56 ng/ml - 100 ng/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (ng/mL)	13.8	37.2	67.3	13.1	36.8	65.7
  St. Dev.	0.39	1.34	3.03	0.77	2.5	4.66
  %CV	2.82	3.60	4.50	5.87	6.79	7.09

• ELISA Kit Component:
  • Lyophilized recombinant human Adiponectin standard: 10 ng/tube x 2
  • Anti-human Adiponectin Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human Adiponectin antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 1.56ng/ml-100ng/ml
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism.1 Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions.2 Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.3 The standard product used in this kit is the product of gene recombination, consisting of 226 (19-244) amino acids with the molecular mass of 36KDa after glycosylation.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum, plasma (heparin, EDTA), tissue homogenates and urine
• Sensitivity: <60 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine
• Reference: 1. Yamauchi, T.; Kamon, J.; Minokoshi, Y.; Ito, Y.; Waki, H.; Uchida, S.; Yamashita, S.; Noda, M.; Kita, S.; Ueki, K.; Eto,
  K.; Akanuma, Y.; Froguel, P.; Foufelle, F.; Ferre, P.; Carling, D.; Nagai, R.; Kimura, S.; Kahn, B. B.; Kadowaki, T. :
  Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase. Nature
  Med. 8: 1288-1295, 2002.
  2. Yokota, T.; Oritani, K.; Takahashi, I.; Ishikawa, J.; Matsuyama, A.; Ouchi, N.; Kihara, S.; Funahashi, T.; Tenner, A. J.;
  Tomiyama, Y.; Matsuzawa, Y. : Adiponectin, a new member of the family of soluble defense collagens, negatively
  regulates the growth of myelomonocytic progenitors and the functions of macrophages. Blood 96: 1723-1732, 2000.
  3. Takemura, Y.; Ouchi, N.; Shibata, R.; Aprahamian, T.; Kirber, M. T.; Summer, R. S.; Kihara, S.; Walsh, K. :
  Adiponectin modulates inflammatory reactions via calreticulin receptor-dependent clearance of early apoptotic bodies. J.
  Clin. Invest. 117: 375-386, 2007.
• Specificity: Natural and recombinant human total Adiponectin
• Immunogen: Expression system for standard: NS0; Immunogen sequence: E19-N244
• Description: For quantitative detection of human Adiponectin in cell culture supernatants, serum, plasma(heparin, EDTA)and urine.

Target Info

• Gene Symbol: ADIPOQ
• Gene Full Name: adiponectin, C1Q and collagen domain containing
• Alias Symbols: 30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP30, ADIPO_HUMAN, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, Adiponectin, Adipoq, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPQTL1, ADPN, apM-1, APM1, C1q and collagen domain-containing protein, GBP28, Gelatin binding protein, Gelatin binding protein 28, Gelatin-binding protein
• NCBI Gene Id: 9370
• Protein Name: Adiponectin
• Description of Target: Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226 (19-244) amino acids with the molecular mass of 36KDa after glycosylation.
• Uniprot ID: Q15848
• Protein Accession #: NP_001171271.1
• Nucleotide Accession #: NM_001177800.1