Adiponectin Antibody (OAPB00319)

Catalog No: OAPB00319

Size:100UG

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Western blot analysis of adiponectin in HL60 cell lysate with adiponectin antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.

Immunohistochemistry of adiponectin in rat brain tissue with adiponectin antibody at 10 μg/mL.

Immunofluorescence of Adiponectin in Rat Brain cells with Adiponectin antibody at 10 ug/mL.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Liquid. Adiponectin Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Positive Control: HL60 cell lysate, rat brain tissue lysate
• Reconstitution and Storage: Adiponectin antibody can be stored at 4C for three months and -20C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Adiponectin antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human adiponectin. The immunogen is located within the last 50 amino acids of Adiponectin.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat, Bovine (71%)
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-Adiponectin antibody is Catalog # OEPB02412
• Application Info: WB: 0.5-2 ug/mL
  IHC: 5 ug/mL
  IF: 20 ug/mL
  Antibody validated: WB in human samples; IHC in mouse & rat samples, IF in mouse and rat samples. All other applications and species not yet tested.
• Reference: Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9.
  ;Tsao T-S, Lodish HF, and Fruebis J. ACRP30, a new hormone controlling fat and glucose metabolism. Euro. J. Pharmacol. 2002; 440:213-21.
  ;Wong GW, Wang J, Hug C, et al. A family of Acrp30/ adiponectin structural and functional paralogs. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.;Shapiro L and Scherer PE. The crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor. Curr. Biol. 1998; 8:335-8.
  

Target Info

• Gene Symbol: ADIPOQ
• Gene Full Name: adiponectin, C1Q and collagen domain containing
• Alias Symbols: 30 kDa adipocyte complement-related protein;ACDC;ACRP30;adipocyte complement-related 30 kDa protein;Adipocyte, C1q and collagen domain-containing protein;adiponectin;adipose most abundant gene transcript 1 protein;adipose specific collagen-like factor;ADIPQTL1;ADPN;APM1;APM-1;GBP28;Gelatin-binding protein;gelatin-binding protein 28.
• NCBI Gene Id: 9370
• Protein Name: Adiponectin|adiponectin precursor [Homo sapiens]
• Description of Target: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
• Uniprot ID: Q15848
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_004788
• Molecular Weight: Predicted: 27 kDa Observed: 26 kDa