Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD02346
Size:96WELLS
Price: $625.00
SKU
OKCD02346
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for ADH1 ELISA Kit (Mouse) (OKCD02346)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)87-101%81-89%80-92%94-104%
EDTA Plasma (n=5)89-98%78-93%92-99%94-101%
Heparin Plasma (n=5)86-103%79-89%87-103%96-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alcohol Dehydrogenase 1 (ADH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alcohol Dehydrogenase 1 (ADH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alcohol Dehydrogenase 1 (ADH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alcohol Dehydrogenase 1 (ADH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)80-9187
EDTA Plasma (n=5)80-10499
Heparin Plasma(n=5)82-9085
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alcohol Dehydrogenase 1 (ADH1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alcohol Dehydrogenase 1 (ADH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ADH1 Microplate96 Wells (12 x 8 Well strips)
ADH1 Lyophilized Standard2
100X Biotinylated ADH1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.133 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Alcohol Dehydrogenase 1 (ADH1). No significant cross-reactivity or interference between Alcohol Dehydrogenase 1 (ADH1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolAdh1
Gene Full Namealcohol dehydrogenase 1 (class I)
Alias SymbolsADH;Adh-;Adh1-;Adh-1;Adh-1-;Adh1-e;Adh-1e;Adh1-t;Adh-1t;Adh-1-t;Adh1tl;Adh3-e;Adh-3e;ADH-A2;ADH-AA;AI194826;alcohol dehydrogenase 1;alcohol dehydrogenase 1 temporal, liver;alcohol dehydrogenase 1, complex;alcohol dehydrogenase 1, electrophoretic;alcohol dehydrogenase 3, electrophoretic;alcohol dehydrogenase A subunit;class I alcohol dehydrogenase.
NCBI Gene Id11522
Protein NameAlcohol dehydrogenase 1
Description of TargetAlcohol dehydrogenase 1
Uniprot IDP00329
Protein Accession #NP_031435.1
Nucleotide Accession #NM_007409.2
Protein Size (# AA)375
Write Your Own Review
You're reviewing:ADH1 ELISA Kit (Mouse) (OKCD02346)
Your Rating
We found other products you might like!