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Datasheets/Manuals | Printable datasheet for ADD1 Antibody (Phospho-Ser726) (OAAF00002) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human ADD1 around the phosphorylation site of Ser726. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | ADD1 (phospho-Ser726) antibody detects endogenous levels of ADD1 only when phosphorylated at serine 726 |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~3000; IHC: 1:50~100; IF: 1:100~1:500; ELISA: 1:40000 |
Reference | Yun Sok Lee, Mol. Cell. Biol., Jan 2007; 27: 438 - 452. Kang Ho Kim, J. Biol. Chem., Dec 2004; 279: 51999 - 52006. Marc Foretz, Mol. Cell. Biol., May 1999; 19: 3760 - 3768. Jae Bum Kim, J. Clin. Invest., Jan 1998; 101: 1. |
Gene Symbol | ADD1|ADD2 |
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Gene Full Name | adducin 1|adducin 2 |
Alias Symbols | ADDA;ADDB;adducin 1 (alpha);adducin 2 (beta);alpha-adducin;beta-adducin;erythrocyte adducin alpha subunit;Erythrocyte adducin subunit alpha;erythrocyte adducin subunit beta. |
NCBI Gene Id | 118|119 |
Protein Name | Alpha-adducin|Beta-adducin |
Description of Target | Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.|Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to the erythrocyte membrane receptor SLC2A1/GLUT1 and may therefore provide a link between the spectrin cytoskeleton to the plasma membrane. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit. |
Uniprot ID | P35611|P35612 |
Protein Accession # | NP_001110.2 |
Molecular Weight | 80 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ADD1 Antibody (Phospho-Ser726) (OAAF00002)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "ADD1 Antibody (Phospho-Ser726) (OAAF00002)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "ADD1 Antibody (Phospho-Ser726) (OAAF00002)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ADD1 Antibody (Phospho-Ser726) (OAAF00002)"?
This target may also be called "ADDA;ADDB;adducin 1 (alpha);adducin 2 (beta);alpha-adducin;beta-adducin;erythrocyte adducin alpha subunit;Erythrocyte adducin subunit alpha;erythrocyte adducin subunit beta." in publications.
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What is the shipping cost for "ADD1 Antibody (Phospho-Ser726) (OAAF00002)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ADD1 Antibody (Phospho-Ser726) (OAAF00002)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ADD1 Antibody (Phospho-Ser726) (OAAF00002)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "80 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ADD1 Antibody (Phospho-Ser726) (OAAF00002)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ADD1|ADD2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ADD1|ADD2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ADD1|ADD2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ADD1|ADD2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ADD1|ADD2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ADD1|ADD2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.