ADCY9 ELISA Kit (Human) (OKCD00837)

Catalog No: OKCD00837 (Formerly GWB-KBBUN3)

Size:96WELLS

Human ADCY9 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase 9 (ADCY9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase 9 (ADCY9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase 9 (ADCY9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adenylate Cyclase 9 (ADCY9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Cyclase 9 (ADCY9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Cyclase 9 (ADCY9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ADCY9 Microplate: 96 Wells (12 x 8 Well Strips)
  • ADCY9 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated ADCY9 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.118 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adenylate Cyclase 9 (ADCY9). No significant cross-reactivity or interference between Adenylate Cyclase 9 (ADCY9) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ADCY9
• Gene Full Name: adenylate cyclase 9
• Alias Symbols: AC9;ACIX;adenylate cyclase type 9;adenylate cyclase type IX;adenylyl cyclase 9;ATP pyrophosphate-lyase 9;type IX ATP pyrophosphate-lyase.
• NCBI Gene Id: 115
• Protein Name: Adenylate cyclase type 9
• Description of Target: Adenylyl cyclase that catalyzes the formation of the signaling molecule cAMP in response to activation of G protein-coupled receptors (PubMed:9628827, PubMed:12972952, PubMed:15879435, PubMed:10987815). Contributes to signaling cascades activated by CRH (corticotropin-releasing factor), corticosteroids and beta-adrenergic receptors (PubMed:9628827).
• Uniprot ID: O60503
• Protein Accession #: NP_001107
• Nucleotide Accession #: NM_001116.3
• Protein Size (# AA): 1353