ADCY10 ELISA Kit (Mouse) (OKCD02343)

Catalog No: OKCD02343

Size:96WELLS

Mouse ADCY10 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase 10, Soluble (ADCY10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase 10, Soluble (ADCY10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase 10, Soluble (ADCY10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adenylate Cyclase 10, Soluble (ADCY10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Cyclase 10, Soluble (ADCY10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Cyclase 10, Soluble (ADCY10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ADCY10 Microplate: 96 Wells (12 x 8 Well strips)
  • ADCY10 Lyophilized Standard: 2
  • 100X Biotinylated ADCY10 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: 0.132 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adenylate Cyclase 10, Soluble (ADCY10). No significant cross-reactivity or interference between Adenylate Cyclase 10, Soluble (ADCY10) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ADCY10
• Gene Full Name: adenylate cyclase 10
• Alias Symbols: s, Sa, sAC, Sacy, 4931412F17, 4930431D04Rik
• NCBI Gene Id: 271639
• Protein Name: Adenylate cyclase type 10
• Description of Target: Catalyzes the formation of the signaling molecule cAMP. May function as sensor that mediates responses to changes in cellular bicarbonate and CO2 levels. Has a critical role in mammalian spermatogenesis by producing the cAMP which regulates cAMP-responsive nuclear factors indispensable for sperm maturation in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. Involved in ciliary beat regulation.
• Uniprot ID: Q8C0T9
• Protein Accession #: NP_766617.2
• Nucleotide Accession #: NM_173029.3
• Protein Size (# AA): 1614